منابع مشابه
Imaging Protein-protein Interactions in vivo
Protein-protein interactions are a hallmark of all essential cellular processes. However, many of these interactions are transient, or energetically weak, preventing their identification and analysis through traditional biochemical methods such as co-immunoprecipitation. In this regard, the genetically encodable fluorescent proteins (GFP, RFP, etc.) and their associated overlapping fluorescence...
متن کاملImaging mRNA and protein interactions within neurons.
RNA-protein interactions are essential for proper gene expression regulation, particularly in neurons with unique spatial constraints. Currently, these interactions are defined biochemically, but a method is needed to evaluate them quantitatively within morphological context. Colocalization of two-color labels using wide-field microscopy is a method to infer these interactions. However, because...
متن کاملNon-invasive imaging of non-linear interactions
Interactions between neuronal assemblies form a basis for the mechanism of functional integration that underlies most of our actions. Studying these interactions is important for understanding the principles of brain operation. However, such neuronal communications are hallmark of a living brain and therefore it is of paramount importance to develop tools for imaging of these interactions on th...
متن کاملImaging molecular interactions in living cells.
Hormones integrate the activities of their target cells through receptor-modulated cascades of protein interactions that ultimately lead to changes in cellular function. Understanding how the cell assembles these signaling protein complexes is critically important to unraveling disease processes, and to the design of therapeutic strategies. Recent advances in live-cell imaging technologies, com...
متن کاملIn Vivo Imaging of Protein-Protein Interactions
Protein-protein interaction (PPI) plays a pivotal role in a wide variety of cellular events and physiological functions, such as enzymatic activity, signal transduction, immunological recognition, DNA repair/replication, among others (Valdar and Thornton, 2001). In addition, biological events that regulate proliferation, differentiation, and inflammation are also commonly mediated through PPI (...
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ژورنال
عنوان ژورنال: Trends in Biotechnology
سال: 2018
ISSN: 0167-7799
DOI: 10.1016/j.tibtech.2017.09.004